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KMID : 1007520090180040971
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Food Science and Biotechnology
2009 Volume.18 No. 4 p.971 ~ p.977
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Inhibition of Oral Epithelial Cell Growth in vitro by Epigallocatechin-3-gallate; Its Modulation by Serum and Antioxidant Enzymes
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Hong Jung-il
Kim Mi-Ri
Lee Na-Hyun
Lee Bo-Hyun
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Abstract
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The most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has been reported to inhibit cell proliferationand induce apoptosis in many types of cancer cells. In the present study, effects of EGCG on the growth of oral epithelial cellsincluding CAL-27 oral squamous carcinoma cells and dysplastic oral keratinocytes (DOK) were investigated. EGCG inhibitedgrowth of CAL-27 cells and DOK with IC50 of 14.4-21.0 and 5.8-14.2μM after 24 and 48hr incubation, respectively. EGCGwas significantly less effective in inhibiting DOK growth. The effects of EGCG, however, were dramatically less pronouncedin the presence of superoxide dismutase (SOD) and catalase. Inhibitory effects of EGCG on CAL-27 cell growth were alsomuch less pronounced in the presence of fetal bovine serum (FBS). EGCG induced caspase-3 activation in both CAL-27 andDOK cells in a serum free condition without SOD/catalase; in the presence of 10% FBS and SOD/catalase, EGCG, even at100μM, did not affect cell growth. The present results indicate that EGCG inhibited oral cell growth with higher potency tomore malignant CAL-27 cells than DOK, and the effects were markedly altered by SOD/catalase and serum content in media.
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KEYWORD
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epigallocatechin-3-gallate (EGCG), oral cell, fetal bovine serum, superoxide dismutase, reactive oxygen species
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